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KMID : 0380019950100030249
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 3 p.249 ~ p.255
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Production of γ-Glutamylcysteine by Immobilized Mixed Microbial System of Recombinant E. coli and Yeast
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Abstract
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y-Glutamylcysteine production by the immobilized microbial system of recombinant Escherichia colt and yeast was investigated. y-Glutamylcysteine was synthesized from L-glutamic acid and L-cysteine in the presence of ATP by the reaction catalyzed by y-glutamylcysteine synthetase. An immobilized microbial cell system was developed for the efficient y-glutamylcysteine production. Recombinant Escherichia coli and yeast were immobilized by alginate. Production of y-glutamylcysteine was better with the recombinant Escherichia colt for both the synthesis of y-glutamylcysteine and the ATP regeneration than the mixed system of recombinant Escherichia colt and yeast. The proper ratio of recombinant Escherichia colt to yeast was experimentary observed to be 1:4 in the mixed system. Although the immobilized system had the slower reaction rate, its reaction stability was increased by 10%.
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